The following is an excerpt from an
article entitled "Cortical barrels: maps and plasticity" by D.J. Simons and P.W.
Land, to appear in Encyclopedia of Life Sciences, Macmillan Reference Ltd.Discovery
of the Cortical Barrel
If ever a picture launched a thousand scientific investigations, it is Figure 15 of the
1970 paper by Thomas Woolsey and Hendrik Van der Loos entitled "The structural
organization of layer IV in the somatosensory region (SI) of mouse cerebral cortex: The
description of a cortical field composed of discrete cytoarchitectonic units".
Figure 15 (slightly adapted) from Woolsey and Van der Loos (1970)
This figure, the last in the report, shows a remarkable correspondence between the
pattern of the mystacial vibrissae (whiskers) on the face of a mouse and the spatial
organization of neuron clusters in its contralateral cerebral cortex. Because of their
characteristic three-dimensional shape, Woolsey and Van der Loos called these neuronal
structures "barrels". Figure 15 presents the authors "one barrel-one
vibrissa" hypothesis which proposed the now firmly established one-to-one
relationship between individual whiskers and their corresponding cortical barrels. The
hypothesis was based in part on work performed by Woolsey several years earlier as a
medical student, in which surface evoked potential recording techniques, perfected during
the 1930's and 1940's by Dr. Clinton Woosley, Thomas father, were used to define the
cortical maps representing visual, auditory and somatic sensory peripheries in the mouse.
In attempting to interpret his physiological findings in the context of previous
anatomical descriptions of the mouse cerebral cortex, Dr. Woolsey realized that the
functional representation of the animals face was coextensive with regions
containing "cell dense nets" described previously by others in what was thought
erroneously to be auditory cortex. Examining this cortical area more closely, Woolsey and
Van der Loos sectioned the cortical tissue in a tangential plane of section that permitted
visualization of a large horizontal expanse of layer IV in a single tissue section. When
viewed from this novel perspective, the pattern of barrels - and their correspondence with
the facial whiskers - was unambiguously revealed. The symbiosis between anatomical and
physiological approaches, so elegantly demonstrated in this early work, has become a
hallmark of the experimental investigation of the whisker/barrel system.
Woolseys and Van der Loos "one barrel-one vibrissa" hypothesis
was quickly confirmed. Influenced by classical neuroembryological experimentation, Van der
Loos and Woolsey (1973) ablated selected whisker follicles in newborn mice and observed,
in the mature animal, an absence of cortical barrels corresponding precisely to the
damaged vibrissae. The diameters of neighboring barrels increased and occupied the
cortical territory that would have represented the ablated whiskers. Importantly, the
effects on the cortical architecture were age-dependent, such that damage to the whisker
follicles led to alterations in barrel field organization only if it was produced within
the first five days of postnatal life. In addition to supporting the "one barrel-one
vibrissa" hypothesis, the neonatal whisker ablation study suggested that the
structural and functional development of the somatosensory cortex was strongly influenced
by the sensory periphery. Compelling support for this idea was provided a few years later
in the form of comparative studies examining the relationship between whiskers and barrels
in different species. These investigations showed that, in those species where cortical
barrels could be observed, their number and spatial organization was always isomorphic to
the pattern of whiskers on the animals face, which varied from one species to the
next. Concurrently, Herbert Killackey demonstrated, using axonal degeneration methods,
that the cortical barrel field received a correspondingly patch-like innervation from the
thalamic ventral posterior medial nucleus, the major pathway in rodents as in higher
mammalian orders that transmits afferent sensory information to the somatosensory cortex.
Direct functional evidence for the "one barrel-one vibrissa" hypothesis was
provided by a series of elegant electrophysiological studies by Carol Welker (1976). Using
microelectrodes to record action potentials from small numbers of neighboring layer IV
neurons in the primary somatosensory cortex of barbiturate-anesthetized rats, she
carefully mapped the representation of the contralateral body and face, making
microlesions at selected recording sites. The cortical hemispheres from these specimens
were then flattened and sectioned in tangential plane, as done earlier by Woolsey and Van
der Loos. Examination of the tissue revealed the presence of barrels or barrel-like
structures within the functionally defined representations of different parts of the
animals body surface, including the fore- and hind-paws. In every case, marker
lesions made at physiologically identified recording sites were found in the appropriate
location within the anatomical map made by the barrels. Moreover, as in other mammalian
sensory systems, the somatosensory representation was grossly distorted, with regions of
high peripheral innervation density, like the hand representation in primates and the
whisker representation in rats, occupying a disproportionately large extent of cortical
space. Shortly thereafter, Simons used more refined electrophysiological techniques to
investigate in rats and mice the receptive field properties of neurons located in
different cortical laminae. These studies demonstrated that a barrel, in layer IV, was the
morphological counterpart of a functional cortical column that extended throughout the
cortical depths and that neuronal receptive field properties varied with laminar location
in a fashion suggestive of a hierarchical organization of information processing. These
early physiological studies thus provided important links to studies in other species,
including cats and monkeys, suggesting important commonalities in the general organization
of sensory cortex and the nature of information processing within local cortical circuits.
The discovery of cortical barrels and the early work that it inspired defined a number
of key issues that have become the focus of subsequent, intense investigation. These
include genetic and epigenetic factors determining the phenotypic expression of barrels,
the nature of centrally directed signals arising from the sensory periphery, the
anatomical and physiological basis of cortical information processing, and the regulation
of cortical structure and function by sensory experience.